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SBA
SBA: 4+ white (Gram pos cocci - S. aureus)
Good isolation. 3 to 4 lines into previous quad. Good feathering at end of each quad.
SBA
SBA
SBA: 4+ white (Gram pos cocci - S. aureus), 4+ large gray mucoid (Gram neg rod - E. coli)
Good isolation, but hit Quad 1 from Quad 4.
SBA
SBA
SBA: 4+ white, Beta and 4+ large gray mucoid
Good isolation of both organisms. Note white pigment from S. aureus along with large zones of hemolysis. Compare to gray mucoid from E. coli.
SBA
SBA
SBA: 4+ white (Gram pos cocci - S. aureus)
Good isolation, but needs more feathering between quads
SBA
SBA
SBA: 3+ translucent Beta (Gram pos cocci - S. pyogenes)
All lines from Quad 2 go into Quad 1. Also hit Quad 1 from Quad 4.
SBA
SBA
SBA: 4+ white (Gram pos cocci - S. aureus)
No isolation, every Quad 2 streak (about 8 or 9) goes into Quad 1. Should have additional lines that feather away from Quad 1. Same with Quad 3.
SBA
4 quadrant streak technique
This is an image of a 4 quadrant streak technique.
Quad 1 is takes up about 1/4 to 1/3 of the plate.
Quad 2 goes into quad 1 two to three times and then feathers out to a point. Quad 2 is the major quad on the plate.
Quad 3 is exactly the same as quad 2, going into the previous quad two to three times and then feathering out to a point near quad 1.
Quad 4 starts at the base of quad 3 and moves out to the center of the plate filling what ever space is left over.